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Dear Sir

I am pleased to introduce myself as M Sc (Agriculture) graduate with specialization in Plant Pathology and willing to work for research and development to share, contribute and further enhance my skills and knowledge.

I look forward to your kind attention and support to build my professional future with your excellence.

I am pleased to attach my Curriculum Vitae for your kind information
Curriculum Vitae
Mr. Sachin Nikam

India


Educational Qualifications:

Examination Passed University/Board Year of Passing Percentage Class
M. Sc. (Plant Pathology) Marathawada Agriculture University Parbhani (India) 2006 87.00 First class with Distinction
B.Sc. (Horticulture) Dr. B S K Agriculture University, Dapoli (India) 2004 81.30 First
H.S.S.C Maharashtra Board, Kolhapur 2000 73.33 First
S.S.C. Maharashtra Board, Kolhapur 1998 64.93 First

Research work carried out for MSc. Degree course

MOLECULAR CHARACTERIZATION OF PAPAYA RINGSPOT VIRUS
Highlights of research MSc. work
Molecular characterization of papaya ringspot virus was carried out for PRSV from Parbhani locality (India).
1)Maintains of PRSV Parbhani isolate was done on papaya, cucumber squash and pumpkin and its’ symptoms were studied.
2)Electron microscopic studies reveled to be flexuous rod shaped particles measuring about 750x12 mm in size.
3)Serological studies were carried out using coat protein specific antibodies using ELISA,
4)Molecular weight of coat protein was approximately 31 Kda using
SDS- PAGE.
5)Coat protein gene of size 1.3 Kb was isolated using PRSV specific primers, which were gel elutated and cloned into pTZ57R plasmid and multiplied in DH5α E coli strain for sequencing.
6)Sequencing coat protein reveled 1479 nucleotide long sequence with specificity of DAG motif at 150th position which is important of aphid transmissibility, and showed 83.5 to 98.2% similarity at nucleotide level with other published Indian isolates.

Work experience:
Presently working as “Research Officer” in Molecular Biology Lab at s Nirmal seeds Pvt. Ltd. Pachora (India) since June 2006 to Till Date
Job responsibilities:
1)Establishment of Molecular Biology lab.
2)Qualitative and quantitative analysis of transgenic plants.
Qualitative and quantitative ELISA analysis of Bt cotton for Cry1Ac, Cry1F gene(Widestrike)
Qualitative ELISA analysis of Bt Brinjal for Vip, Cry2Ab gene.
Qualitative ELISA analysis of Bt Okra for Cry1Ac gene.
3)PCR analysis of transgenic plants.
Analysis of transgenic Cotton for Cry1Ac, trangenic Brinjal for Vip gene.
4)Blotting analysis to find out copy number of inserts in transgenic plants.
Southern Blotting analysis of transgenic Brinjal to find out copy number of Vip gene.

Award/scholarship
1) Awarded merit scholarship for Bachelor Degree program.
2) Ranked second for Prof. M.J. Narsimhan Academic merit award contest during National Symposium of Indian Phytopathological Society (West Zone) held at NBSS & LUP Nagpur on November 2006


Workshops, Seminar & Training programs attended
1) Attended IInd Global Conference organized by Indian Society of Mycology and Plant pathology in November 2005 at Udaypur (Rajasthan) and presented two research papers in poster presentation session as fallow
-Studies on physical properties of virus causing stunting in Pea
-Transmission of virus causing stunting in Pea
2) Attended Annual National Symposium on “Crop disease management in dry land agriculture “organized by Indian Phytopathological Society in January 2005, at Parbhani (Maharashtra)
3) Attended summer training program on “Molecular characterization and diagnostic techniques for plant viral and fungal pathogens” at National Research Center for Banana ( Trichy) in May 2006

Personal Information
Name: Sachin Nikam
Father’s Name: Balasaheb Nikam
Date of Birth: 21.02.1983
Age: 25 Yrs.
Sex: Male Marital Status: Single


REFERENCES
1) Dr K. T. Apet
Director of Student Welfare
Marathawada Agriculture University, Parbhani (M.S)
Mobile No: +91 9421461895 Email:ktapet123@rediffmail.com

2 ) Dr. M. Krishna Reddy,
Senior Scientist,
Indian Institute of Horticulture Research,
BANGALORE -65 Karnataka State, India
Tel: +918028466420(Extension 272) Email: mkreddy@vsnl.com

3) Mr. F. S. Patil
Associate Professor,
Department of Biotechnology
Marathawada Agriculture University, Parbhani (M.S)
Mobile No: +91 9422924924




Thanking you

With warm regards

Sincerely Yours

Mr. Sachin Nikam