As so many people ask about the subject of growing mushrooms I decided to take the time to write you a good explanation.
It will take some time for the article to be finish, I will write some more info when i can.
Please do not copy the article!
Growing mushrooms by shachar alfie
Mushroom can be classified to three types on the base of the role of mushrooms in the ecosystem.
The classes are Mycorrhizal, saprophytic and parasitic, the mycorrhizal mushroom live in symbiosis with the root of a plant, the parasitic mushroom as you can guess by the name live with parasitic relationship to a plant and the saprophytic mushroom is the recycler of the ecosystem, and as you can guess most cultivated mushrooms are saprophytic.
The saprophytic mushroom as a recycler needs an organic material to recycle back to the ecosystem.
The recycled material needs to be moistened to 65% water for sawdust base substrate or 70 to 75% for straw.
List of cultivated saprophytic mushrooms that will grow on straw
Pleurotus ostreatus -Florida Oyster
Pleurotus ostreatus var. columbinus -Blue Oyster Mushroom
Pleurotus pulmonarius -Summer Oyster Mushroom
Pleurotus cornucopiae -Yellow Oyster Mushroom
Pleurotus eryngii -King Oyster Mushroom
Pleurotus salmoneostramineus -Pink Oyster Mushroom
Volvariella volvacea -Paddy Straw Mushroom
Calocybe indica -Milky mushroom
List of cultivated saprophytic mushrooms that will grow on sawdust
Agrocybe aegerita -Black Poplar Mushroom
Pholiota nameko -Nameko Mushroom
Flammulina velutipes -Enoki
Hypsizygus ulmarius -Elm Oyster Mushroom
Hypsizygus tessulatus Buna-Shimeji
Lentinula edodes -Shiitake Mushroom
Hericium erinaceus -Monkey Head, Lion’s Mane
Ganoderma lucidum -Reishi
Pleurotus eryngii -King Oyster Mushroom
Mushrooms that will grow on (or recycle) straw or wood/sawdust are primary decomposers.
Button and Portobello mushrooms for example are secondary decomposer as they continue the job of the compost microorganisms.
To be continue!
Me after finding morels, morels are some of the most expensive mushrooms in the world
Last edited by mushroomsil; June 25th, 2009 at 09:01 PM.
The role of the lab is to expend the mycelium from the small mycelium mass in the test tubes to many spawn bags.
To inoculate 1 ton of straw (dry weight) you will need in the range of 50 to 100 KG of spawn.
The lab is an easy to clean room, in the room you will find A laminar flow hood (a big hepa filter connected to a blower), positive air pressure generator, autoclave, shelves, Petri dishes, jars, test tubes, lab blender and a magnetic stirrer.
The grow rooms:
Three very important phases will happen in the grow room:
Incubation, Primordia formation and Fruit body development.
The grow room is a very big easy to clean room; in the room you will find a cooling system, fogger, air circulation system, a good air filter, fluorescent lamps.
The ingredients are:
1 ton of straw (cereal straw)
50 KG of oyster spawn
The tools are:
Blower fan + Manual Speed Control
Fogger + Humidistat
Electrostatic\HEPA Air Filter
Wet the dry straw to 70 to 75 % water, and use the Steam generator to expose the wet straw to a temp of 64C to 71C for 2 hours.
Let the straw cool to 25C to 30C and mix the straw and the oyster spawn(50 KG of spawn-"wet weight" to 1 ton of "dry weight" straw)
cooling the straw
Fill bags with the spawned straw.
Punch holes in the bags (for a 2.5 meter high, 30CM diameter bag you need 200 to 300 holes)
Install the Blower fan + Manual Speed Control / Fogger + Humidistat / Electrostatic\HEPA Air Filter\ fluorescent daylight in the grow room.
Let the bags in too the grow room.
Set the Humidistat to 95% RH and the Blower speed to low.
Close the light!!!
Let the bags incubate for 7 to 10 days.
After the incubation the straw will be white from the mushroom mycelia.
the bags after the incubation
Set the Humidistat to 95% RH and the Blower speed to high.
Open the light!!! (You will need 12 hours light on / 12 hours light off)
in 3 to 5 days you will see the primordia formation (small mushrooms)
at the end of the primordia formation set the Humidistat to 90% to 85% RH
let the mushroom grow for 3 to 5 more days and you can harvest the mushrooms.
mushrooms for harvest
Set the Humidistat to 95% RH and in 3 to 5 days you will see the new primordia formation :-)
You can harvest a new crop every 7 to 14 days for three to four crops.
To be continue.....
Last edited by mushroomsil; July 23rd, 2009 at 07:19 AM.
Before I will explain to you how to make spawn I will need to answer the question what is spawn.
Spawn is an organic material colonized by the mushroom mycelium, usually cereal grain /wood sawdust (and sometime wood plugs for log cultivation).
Spawn is used to inoculate the substrate that is used for the production of mushrooms, the idea behind spawn is that each individual cereal grain or a small piece of sawdust will perform as a separate mycelium inoculation point.
The more inoculation points the faster the mycelium will colonize the substrate.
By using spawn what we are actually doing is asexual reproduction of the mushroom, or in a more simple word cloning.
The advantage of using cloning and spawn is that you know exactly what you're getting, the new offspring will be genetically identical to their single parent, while in using sexual reproduction from spores every new offspring will have a different genotype.
The first stage for making spawn is buying a clone of the mushroom you want to grow.
You can buy a clone/mushroom culture from a company that have a culture library collection.
What you will actually get is a test-tube; in the test tube you will find a jelly from agar, nutrient, and a growing mushroom mycelium.
mushroom culture test-tube
The second stage is to make Petri dishes with sterilized agar solution.
A very simple way to make a solution is to cook potatoes in water for one hour.
Now you throw the potatoes but don't throw the water!
Mix the water with the appropriate amount of agar powder put the mixed solution in a bottle and put the bottle in the autoclave.
a small autoclave picture
To the autoclave add glass Petri dishes.
Sterilize the bottle and Petri dishes for 30 minutes at 15 psi.
After the sterilization let the autoclave cool down to 0 psi in front of the HEPA filter.
Open the autoclave take out the bottle and Petri dishes.
Always work in front of the laminar flow hood (HEPA filter).
pour the agar solution in to the Petri dishes and let the solution to cool and harden.
The Petri dishes with the sterilized agar solution are ready.
laminar flow hood picture
To be continue.....
Last edited by mushroomsil; August 11th, 2009 at 12:08 PM.